Search results for "Cucumis sativus"
showing 10 items of 10 documents
Dynamics and risk assessment of pesticides in cucumber through field experiments and model simulation
2020
Abstract Pesticides are often applied multiple times during cucumber cultivation in China. In order to obtain the residue concentrations and subsequently human health risk assessment after pesticide multiple applications, plenty of field trials have been conducted, consuming a lot of labor force and funds. The application of kinetic models can address this problem to some extent by predicting the residue values of pesticides in cucumber. In this study, a dynamic model (dynamiCROP) was applied in combination with field experiments to investigate the distribution, translocation, and dissipation after the one-time application of seven pesticides in a cucumber-soil environment. Moreover, the re…
Two-stage anaerobic digestion of tomato, cucumber, common reed and grass silage in leach-bed reactors and upflow anaerobic sludge blanket reactors
2010
Abstract Anaerobic digestion of tomato, cucumber, common reed and grass silage was studied in four separate two-stage reactor configuration consisting of leach bed reactor (LBR) and upflow anaerobic sludge blanket reactor (UASB). LBR studies showed that COD solubilization for cucumber and grass silage was higher (50%) than tomato (35%) and common reed (15%). Results also showed that 31–39% of initial TKN present in tomato and cucumber was solubilized in the leachates and 47–54% of the solubilized TKN was converted to NH4-N. The corresponding values for common reed and grass silage were 38–50% and 18–36%, respectively. Biomethanation of the leachates in UASB reactors resulted in methane yiel…
Application of Toxkit microbiotests for toxicity assessment in soil and compost
2004
The potential of Toxkit microbiotests to detect and analyze pollution in agricultural soil and the quality of compost was studied. The toxicity tests used included seed germination biotests using cress salad (Lepidum sativum L.), tomato (Lycopersicum esculentum L.), and cucumber (Cucumis sativus L.), and the Toxkit microbiotests included those with microalgae (Selenastrum capricornutum), protozoa (Tetrahymena thermophila), crustaceans (Daphnia magna, Thamnocephalus platyurus, and Heterocypris incongruens), and rotifers (Brachionus calyciflorus). Experiments on compost were undertaken in a modified solid-state fermentation system (SSF) and under field conditions (in a windrow). To promote th…
Cucumis sativus l. Seeds ameliorate muscular spasm-induced gastrointestinal and respiratory disorders by simultaneously inhibiting calcium mediated s…
2021
Cucumis sativus L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a C. sativus seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. C. sativus seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin …
LC-MS/MS characterisation and determination of dansyl chloride derivatised glyphosate, aminomethylphosphonic acid (AMPA), and glufosinate in foods of…
2021
Abstract Glyphosate and other polar and acidic pesticides have been particularly studied due to the concerns over widespread and intensive use. The chemical properties of these compounds necessitate use of customised methods, such as derivatisation or ion exchange chromatography. These approaches present a compatibility problem with ESI-MS due to presence of salts and non-volatile compounds. For that reason, a simple procedure has been developed for the extraction, pre-column derivatisation with dansyl chloride (5-(dimethylamino)naphthalene-1-sulfonyl chloride), and mass spectrometric detection of glyphosate, AMPA, and glufosinate after the separation on a C18 stationary phase. The dansyl d…
Design and development of heterologous competitive immunoassays for the determination of boscalid residues
2014
Boscalid is a modern agrochemical belonging to the so-called chemical class of succinate dehydrogenase inhibitor fungicides. With the aim of developing rapid analytical screening methods for this relevant compound, we herein report the synthesis of new boscalid mimics and the study of their suitability for the production of polyclonal antibodies. Aliphatic spacer arms equivalent in length and composition were tethered at two different aromatic rings of the target molecular structure. These haptens, besides being used for immunization, were employed in the development of heterologous competitive enzyme-linked immunosorbent assays (cELISAs) in order to improve assay detectability. Direct and …
Crotonic acid as a bioactive factor in carrot seeds (Daucus carota L.)
2004
Water extracts from the carrot seed (Daucus carota L.) var. Perfekcja exhibit plant growth inhibitory properties against cress, cucumber, onion and carrot in a dose-dependant manner. This property results from the action of low-and high-molecular components of the extract. The low-molecular component was identified as crotonic acid ((E)-2-butenoic acid). Its presence was also confirmed in other late varieties of carrot. The determined strong herbicidal properties of crotonic acid and its availability after release to soil combined with its high level in seeds suggest that it might be considered as an allelopathic and autotoxic factor in the seeds.
Sensitive Monoclonal Antibody-Based Immunoassays for Kresoxim-methyl Analysis in QuEChERS-Based Food Extracts
2014
Kresoxim-methyl is nowadays widely used to combat a diversity of common diseases affecting high-value crops. In this article, we report the development and characterization of two novel immunoassays for the analysis of this pioneer strobilurin fungicide, and for the first time, a validation study with food samples was performed. A direct and an indirect competitive immunoassay based on a new anti-kresoxim-methyl monoclonal antibody were developed for sensitive and specific chemical analysis. Optimized assays showed limits of detection of 0.1 μg/L. Fruit and vegetable samples were extracted with acetonitrile by the QuEChERS procedure and analyzed by the developed immunoassays after a simple …
Pythium perplexum isolated from soil in France: morphology, molecular characterisation and biological control.
2001
Pythium perplexum (F-926) was isolated from a soil sample taken in the Burgundy region in France. In 1907, it was mistakenly described by Bulter as P. vexans. Despite morphological resemblance, the comparison between the internal transcribed spacer 1 regions of the ribosomal DNA of the two fungi leaves no doubt of their different identities. P. perplexum was found to be highly pathogenic to cucumber seedlings. Damping-off disease of cucumber caused by P. perplexum can effectively be controlled by using the soil bacterium Serratia plymuthica (B-781). The details of the morphology and the molecular characterisation of P. perplexum and its biological control with S. plymuthica are described he…
Metabolic Study of Cucumber Seeds and Seedlings in the Light of the New, Controversial Trend of Preventive Use of Systemic Fungicides
2023
Cucumber is one of the most commonly produced vegetable crops. The greatest economic losses in the yields of these crops have resulted from fungal infections—powdery mildew and downy mildew. The action of fungicides not only affects the fungi, but can also lead to metabolic disorders in plants. However, some fungicides have been reported to have positive physiological effects. Our research focused on the action of two commercially available fungicides, Scorpion 325 SC and Magnicur Finito 687,5 SC, on plant metabolism. Two approaches were used to check the effect of the fungicides at the early stage of plant development when metabolic changes occur most dynamically: spraying on the leaves of…